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Initial workflow, Took three technical replicates and compared tot indep spectra

Data from a high-CO2 individual

Simply took max spectral count in pivot table; if multiple Gigasdatabase in cell, only took first (reason: determined they were in alphabetical order therefore assuming consistency across samples.

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Next step -

Examine technical replicate #1 from 4 high CO2 and 4 ambient oysters

101B_2_01 (above)

101B_5_01 done

101B_8_01 done 

Screen Recording: http://aquacul4.fish.washington.edu/~steven/armina/ProteinAnalyisis_crude.mov

101B_11_01 done 

Ambient

103B_221_01 done (as are other replicates

103B_224_01 done

103B_227_01 done

103B_230_01 done

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Create Master working spreadsheet

Included counts of 8 runs (separate spreadsheets

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Ambient Oysters joined

of note 576 unique proteins expressed

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556 unique proteins in Hi Co2 (noting only looking at those with at least 4 spectral counts)

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note 2fold only included those proteins expressed in both.

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OA_DEG_w_GO.xls

GO terms (with at least 10 proteins) from all 705 proteins (2 off chart)

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580 unique SPID for all Proteins

314 unique SPID for DEG (DEP)

DAVID

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OA_DEG_w_GOslim.xlsx

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OA_DEG_w_GOslim_GeneName.xlsx

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OA_ONLY_Proteins.txt

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data

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REVIGO

OA UPREGULATED (expressed in both)

OADOWN

OAonly

slightly less terms

OA only Molecular Function --- size is expression level

OA ONLY size is expression level